Avtor/Urednik		Gudović, R
Naslov		Stereological analysis of the inferior olivary nucleus in the developing human brain
Tip		članek
Vir		Acta Stereol
Vol. in št.		Letnik 12, št. 2
Leto izdaje		1993
Obseg		str. 305-10
Jezik		eng
Abstrakt		The stereoloogical analysis of the inferior olivary nucleus was performed on 25 human fetal brains aged from 8th to 31st gestation week (GW) and on the brain of a newborn. The fetal breins were obtained on hysterectomy due to myoma uteri or after spotaneous abortion because of cervical incopetency. The brains were fixed in 10% formalin solution, embedded in celloidin and paraffin and cut in frontal plane in 6, 15 and 30/um and stained with cresyl iolet. The stage of maturation of the nerve cells was established on the basis of the degree of basophilia, metachromatic characteristic of the nucleoli and colouring of the cytoplasm and the occurrence of the Nissl bodies. In all of the investigated stages the diameter of the nerve cell nuclei was determined by the eye-piece graticule. Stereological analysis was performed by using Weibel's M 42 test system. The results obtained indicate that the nerve cells of the second stage of maturation were observable up to the 16.5th GW, and the cells of the third stage from l7.5th to 22nd week GW. Parallely with cells of the third stages of maturation in the 22nd GW the cells of the fourth stage were conspicuous until birth. The volume of the nerve cell nuclei was increasing until birth. A rise in average olumes was statistically significant (p less th.0.001). The value of the numerical density of the nerve cell nuclei was decreasing in the course of development. The decreaseof the numarical density of the nerve cell nuclei was statistically significant up to the l6.5th GW. Between the l6.5th and l9.5th GW the decrease of the numerical density of the nerve cell nuclei was statistically insignificant Since l9.5th GW until the birth the decrease of the umerical density of the nerve cell nuclei was, again statistically significant (p less th.0.001).
Deskriptorji		BRAIN OLIVARY NUCLEUS INFANT, NEWBORN HISTOLOGICAL TECHNIQUES CELL COUNT