Avtor/Urednik		Kolundzic, Nikola; Khurana, Preeti; Devito, Liani; Jeriha, Jakob; Marx, Jennifer; Hobbs, Carl; Wong, Xuan Fei Colin Cornelius; Common, John E. A.; Liović, Mirjana; Ilic, Dusko
Naslov		Induced pluripotent stem cell line heterozygous for p.R501X mutation in filaggrin
Tip		članek
Vol. in št.		, št. Vol. 39
Leto izdaje		2019
Obseg		str. 1-6
ISSN		1876-7753 - Stem cell research
Jezik		eng
Abstrakt		We have generated an induced pluripotent stem cell (iPSC) line KCLi003-A (iOP101) from epidermal keratinocytes of a female donor, heterozygous for the loss-of-function mutation p.R501X in the filaggrin gene (FLG), using non-integrating Sendai virus vectors. Derivation and expansion of iPSCs were performed under xeno-free culture conditions. Characterization and validation of KCLi003-A line included molecular karyotyping, mutation screening using restriction enzyme digestion, next generation sequencing (NGS), while pluripotency and differentiation potential were confirmed by expression of associated markers in vitro and by in vivo teratoma assay.
Proste vsebinske oznake		matične celice filargin mutacija stem cell filaggrin mutation