Avtor/Urednik		Mikuletič, Tina; Steyer, Andrej; Kotar, Tadeja; Zorec, Tomaž Mark; Poljak, Mario
Naslov		A novel reassortant mammalian orthoreovirus with a divergent S1 genome segment identified in a traveler with diarrhea
Tip		članek
Vol. in št.		, št. Vol. 73
Leto izdaje		2019
Obseg		str. 378-383
ISSN		1567-1348 - Infection, genetics and evolution : Journal of molecular epidemiology and evolutionary genetics in infectious diseases
Jezik		eng
Abstrakt		Mammalian orthoreoviruses with reassortant genomes have recently been detected in various mammals and humans with respiratory, central nervous system, and gastrointestinal symptoms. This study describes the detection of the novel reassortant mammalian orthoreovirus SI-MRV07 in a traveler with gastroenteritis that returned from southeast Asia. The virus was initially detected with electron microscopy in stool, followed by propagation in the epithelial-like monkey kidney Marc145 cell line. Whole-genome sequencing revealed the reassortant nature of the genome segments, where by the S1 genome segment was the most variable according to known sequences deposited in GenBank. Based on the nucleotide sequence of the S1 genome segment, the isolate clusters to serotype 2, close to the reference strain Jones T2J. The patient's serum showed the highest virus neutralization capacity toward SI-MRV07 and T2J isolates. This study provides additional insight into emerging mammalian orthoreoviruses with reassortant genomes and possible zoonotic potential, which should be carefully monitored in the future.
Proste vsebinske oznake		mammalian orthoreovirus human S1 genome segment ortoreovirus sesalcev človek segment genoma S1