Avtor/Urednik		Alibegović, Armin; Blagus, Rok; Zubiavrre Martinez, Inigo
Naslov		Safranin O without fast green is the best staining method for testing the degradation of macromolecules in a cartilage extracellular matrix for the determination of the postmortem interval
Tip		članek
Leto izdaje		2019
Obseg		str. str.
ISSN		1547-769X - Forensic science, medicine, and pathology
Jezik		eng
Abstrakt		Methods for the determination of the postmortem interval (PMI) include methods that monitor the postmortem changes of cellsand molecules in different tissues. The rate of pathological degradation of macromolecules in the extracellular matrix (ECM) ofhyaline cartilage could be verified by assessing the intensity of collagen and proteoglycan (PG) staining. In the presented in vitropilot study, this methodology was used for the first time to determine PMI. The osteochondral samples of three donors werestored at 11 °C and 35 °C and analyzed on day 1, day 12, and day 36 postmortem. The intensity of staining using Masson's trichrome and Sirius red for collagen, and Alcian blue and Safranin O dyes for PG was estimated ten times according to themodified Bern grading scale. Statistical analysis showed that the Safranin O without Fast green method is the most appropriate(raters agreement 0.5541) for up to 36 days postmortem, and that the influence of time is more important (p=0.023)thantheinfluence of temperature (p= 0.061) on the degradation of the ECM macromolecules. The described method, which is simple andcan be performed in any histological laboratory, should be verifiedin corporeconditions, on a large number of donors, and usingan objective method for assessing the intensity of cartilage macromolecule staining for PMI determination.
Proste vsebinske oznake		postmortem changes hyaline cartilage extracellular matrix posmrtne spremembe hialinski hrustanec izvencelični matriks