Avtor/Urednik		Čamernik, Klemen; Mihelič, Anže; Mihalič, Rene; Marolt, Darja; Janež, Andrej; Trebše, Rihard; Marc, Janja; Zupan, Janja
Naslov		Increased exhaustion of the subchondral bone-derived mesenchymal stem/ stromal cells in primary versus dysplastic osteoarthritis
Tip		članek
Leto izdaje		2020
Obseg		str. str.
ISSN		2629-3277
Jezik		eng
Abstrakt		Mesenchymal stem/ stromal cell (MSC) exhaustion has been suggested to be a hallmark of aging. Osteoarthritis has a complexetiology that comprises several factors. Dysplasia has been shown to be an individual risk factor for osteoarthritis. Subchondralbone changes are often the first detectable alterations in osteoarthritis. In this study, we aimed to determine whether skeletalMSCs are differentially affected in patients with primary versus dysplastic osteoarthritis. Patients undergoing hip arthroplasty dueto primary osteoarthritis (n= 11) and osteoarthritis with hip dysplasia (n= 10) were included in the study. Femoral headsubchondral bone was used for isolation of MSCs. The cells were compared using detailed ex-vivo and in-vitro analyses, whichincluded immunophenotyping, colony-forming-unit fibroblast assay, growth kinetics, senescence, multilineage potential,immunophenotyping, and MSC marker-gene expression profiling. Isolated cells from primary osteoarthritis patients showeddecreased viability in comparison with those from dysplasia patients, with similar mesenchymal fractions (i.e., CD45/ CD19/CD14/ CD34-negative cells). In-vitro expanded MSCs from primary osteoarthritis patients showed reduced osteogenic andchondrogenic potential in comparison with dysplasia patients. There were no differences in clonogenicity, growth kinetics,senescence, adipogenic potential, and immunophenotype between these groups. Gene expression profiling showed well-known marker of bone marrow MSCs, the leptin receptor, to be significantly lower for primary osteoarthritis patients. Our studyshows that the pathology of primary osteoarthritis is accompanied by bone MSC exhaustion, while biomechanical dysfunctionassociated with hip dysplasia can induce secondary osteoarthritis without this MSC impairment. Our study suggests thatsubchondral bone MSC exhaustion is implicated in the pathology of primary osteoarthritis.
Proste vsebinske oznake		mesenchymal stem/ stromal cells primary osteoarthritis hip dysplasia mesenhimske matične / stromalne celice primarni osteoartritis displazija kolka