Avtor/Urednik		Plemenitaš, Ana
Naslov		Sensing and responding to hypersaline conditions and the HOG signal transduction pathway in fungi isolated from hypersaline environments
Tip		članek
Vol. in št.		Letnik 7, št. 11
Leto izdaje		2021
Obseg		str. 1-13
ISSN		2309-608X
Jezik		eng
Abstrakt		Sensing and responding to changes in NaCl concentration in hypersaline environments is vital for cell survival. In this paper, we identified and characterized key components of the high-osmolarity glycerol (HOG) signal transduction pathway, which is crucial in sensing hypersaline conditions in the extremely halotolerant black yeast Hortaea werneckii and in the obligate halophilic fungus Wallemia ichthyophaga. Both organisms were isolated from solar salterns, their predominating ecological niche. The identified components included homologous proteins of both branches involved in sensing high osmolarity (SHO1 and SLN1) and the homologues of mitogen-activated protein kinase module (MAPKKK Ste11, MAPKK Pbs2, and MAPK Hog1). Functional complementation of the identified gene products in S. cerevisiae mutant strains revealed some of their functions. Structural protein analysis demonstrated important structural differences in the HOG pathway components between halotolerant/halophilic fungi isolated from solar salterns, salt-sensitive S. cerevisiae, the extremely salt-tolerant H. werneckii, and halophilic W. ichthyophaga. Known and novel gene targets of MAP kinase Hog1 were uncovered particularly in halotolerant H. werneckii. Molecular studies of many salt-responsive proteins confirm unique and novel mechanisms of adaptation to changes in salt concentration.
Proste vsebinske oznake		halotolerantne/halofilne glive Hortaea werneckii Wallemia ichthyophaga halotolerant/halophilic fungi Hortaea werneckii Wallemia ichthyophaga