Avtor/Urednik		Rohozinski, Jan; Hancock, John M.; Keniry, Max A.
Naslov		Polycytosine regions contained in DNA hairpin loops interact via a four-stranded, parallel structure similar to the i-motif
Tip		članek
Vol. in št.		Letnik 22, št. 22
Leto izdaje		1994
Obseg		str. 4653-4659
ISSN		0305-1048 - Nucleic acids research
Jezik		eng
Abstrakt		Thermal denaturation profiles of an oligodeoxynucleotide that forms a hairpin structure with a cytidine-rich loop show an unexpected transition at 60 degrees C at pH 5.0 but not at pH 8.0. Analytical ultracentrifugation shows that this transition reflects dimer formation via the interaction of loops from two molecules to form a novel structure termed the h-dimer. The dependence of this structure on low pH implies the formation of cytosine-protonated cytosine base pairs. NMR spectroscopy, thermal denaturation and ultraviolet absorption spectral analysis suggest a similarity to the i-motif structure recently proposed for the interaction of deoxycytidine oligomers. The use of hairpin loops to form i-motif-like structures may prove useful in searches for cognate proteins and possibly in the production of antibodies.
Proste vsebinske oznake		policitozinske regije zanke DNK vzporedna struktura polycytosine regions DNA hairpin parallel structure