Avtor/Urednik     Batista, Urška; Šentjurc, Marjeta
Naslov     EPR study of the sea anemone cytolysin, equinatoxin II, cytotoxicity on V-79 cells
Tip     članek
Vir     Cell Biol Int
Vol. in št.     Letnik 19, št. 3
Leto izdaje     1995
Obseg     str. 215-22
Jezik     eng
Abstrakt     Electron paramagnetic resonance (EPR) was used to study the effect of equinatoxin (EgT II), a cytolytic protein isolated from the sea anemone Actinia equina L., on membrane fluidity and cell metabolism of V-79 cells; the reduction of the spin probe incorporated into the cell membranes as well as the oxygen consumption in the cell suspension were measured. The results were compared with the results obtained by the cell viability study. Under the influence of EgT II (less than 37.5 micro g/10 6 cells) no significant changes in cell membrane fluidity were observed, while reduction kinetics of the spin probe and the oxygen consumption decreased when the cells were kept in Tris buffer solution. However, in the presence of 10 percent fetal calf serum, which prevented cell lysis, the effects of EgT II were diminished. The oxygen consumption corresponds to the cell viability changes but the reduction kinetics alterations indicate that some oxidation-reduction processes other than cell respiration are affected by EqT II in the absence of serum. The effect seems to be indirect, probab;y due to the formation of pores which are associetd with changes permeability of plasmalemma for metabolites and ions.
Deskriptorji     CYTOTOXINS
MEMBRANE FLUIDITY
CELLS, CULTURED
OXYGEN CONSUMPTION
CRICETULUS
ELECTRON SPIN RESONANCE SPECTROSCOPY
SEA ANEMONES