Avtor/Urednik		Bedenk, Jure; Režen, Tadeja; Jančar, Nina; Geršak, Ksenija; Virant-Klun, Irma
Naslov		Effect of in vitro maturation of human oocytes obtained after controlled ovarian hormonal stimulation on the expression of development- and zona pellucida-related genes and their interactions
Tip		članek
Leto izdaje		2022
Obseg		str. str.
ISSN		1933-7191 - Reproductive sciences (Thousand Oaks, Calif.)
Jezik		eng
Abstrakt		In an in vitro fertilization program, approximately 10‐15% of oocytes obtained after controlled ovarian stimulation are immature, with germinal vesicles (GVs). These oocytes are usually discarded in clinical practice; however, an in vitro maturation (IVM) procedure can be applied to mature them. There are scarce data in the literature on the effect of IVM on the expression of important development- and zona pellucida (ZP)‐related genes in human oocytes; therefore, we wanted to determine this. One hundred nine human oocytes were collected from patients enrolled in an intracytoplasmic sperm injection program. The expression of the BMP4, GDF9, ZP1, ZP2, ZP3, and ZP4 genes was analyzed using RT‐qPCR in oocytes matured in vitro with different reproductive hormones in the IVM medium (AMH, FSH + hCG, FSH + hCG + AMH), in in vivo matured oocytes and in immature oocytes with GVs. No statistically significant differences in the expression of selected genes in oocytes were observed among groups with different reproductive hormones in IVM medium. However, several interesting significant correlations were found between BMP4 and GDF9, and ZP1 and ZP4; between GDF9 and ZP1, and ZP2 and ZP4; and between ZP1 and ZP3 and ZP4 in the in vitro matured oocytes, while no such correlations were present in other groups of oocytes. The type of reproductive hormone in the maturation medium does not affect the expression of the analyzed genes in oocytes during the maturation process. However, the in vitro maturation procedure itself generated correlations among analyzed genes that were otherwise not present in in vivo matured and immature oocytes.
Proste vsebinske oznake		human oocytes ovarian hormonal stimulation development genes človeške jajčne celice hormonska stimulacija jajčnikov razvojni geni