Avtor/Urednik		Hoerner, M; Maly, R; Soeltz-Szoets, J
Naslov		Recent advances in the diagnosis of genital HPV infections
Tip		članek
Vir		Acta Dermatovenerol Alp Pannon Adriat
Vol. in št.		Letnik 4, št. 3
Leto izdaje		1995
Obseg		str. 89-93
Jezik		eng
Abstrakt		Genomic typing is considered the method of choice for detection of genital infections with human papillomaviruses. Most assays are based on determination of DNA:RNA-hybrids by autoradiographic or enzymatic staining. According to our results, the sensitivity of a conventional dot-blot method was limited by the amount of cellular substrate gained for testing. In situ-hybridization applied to cytologic swabs was not useful, while applied to histological samples it allows direct assessment of the epithelial affection. Polymerase chain reaction dramatically increases the sensitivity and specificity of subsequent hybridization tests. It can be used in selected cases. Southern blot assays and other highly specific tests are exclusively performed to answer scientific questions. In contrast to that, a relatively new chemoluminescence assay can be recommended for routine use. It allows the differentiation between two viral groups of different malignant risk as well as the detection of single viral strains. Compared to other routine methods, the chemoluminescence assay combines the advantage of easy handling with sensitivity in the high and low risk spectrum of viral strains.
Deskriptorji		PAPOVAVIRIDAE INFECTIONS CERVIX POLYMERASE CHAIN REACTION IMMUNOBLOTTING IN SITU HYBRIDIZATION