Avtor/Urednik		Lah, T; Vihar, M; Dubbin, A; Turk, V
Naslov		Horse alpha2-macroglobulin: circular dichroism studies of conformational changes upon reaction with proteinases and methylamine
Tip		članek
Vir		Biol Chem Hoppe Seyler
Vol. in št.		Letnik 368, št. 5
Leto izdaje		1987
Obseg		str. 487-92
Jezik		eng
Abstrakt		The interaction of horse alpha2-macroglobulin with methylamine, trypsin and cathepsin D was studied by circular dichroism in the far and near UV region, by polyacrylamide gel electrophoresis and by determination of its inhibitory activity. The CD spectra of horse alpha2-macroglobulin resemble those of bovine and human alpha2-macroglobulin. The CD spectra were changed in a different manner after the interaction of alpha2-macroglobulin with methylamine, trypsin and inactive or active cathepsin D, indicatinig that more than one conformational change occurs. Cathepsin D activity was not affected by complex formation with horse alpha2-macroglobulin. In contrast to the action of trypsin, treatment with methylamine did not increase the electrophoretic mobility of alpha2-macroglobulin.
Deskriptorji		HORSES MACROGLOBULINS CATHEPSINS TRYPSIN CIRCULAR DICHROISM