Avtor/Urednik		Knežević, M; Alscher, M; Žunec, P; Hartman-Pretnar, K; Rožman, P; Čurin-Šerbec, V
Naslov		Cultivation of mouse-mouse hybridomas producing IgM monoclonal antibodies
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 431, št. 6 Suppl 2
Leto izdaje		1996
Obseg		str. R231-2
Jezik		eng
Abstrakt		The mouse-mouse hybridomas producing monoclonal antibodies (MAbs) for ABO blood group determination were cultivated for 10 days in 25 cm/2 Roux bottles using standard cultivation medium (DMEM) with different foetal-calf serum (FCS) concentrations (2-13%). The highest specific production rates (200-1100 micro g/10/6 cells/day) for MAbs were measured at the end of the cultivation: this phenomenon could be explained by advanced cell death and liberating the content of the cells into the medium.
Deskriptorji		ANTIBODIES, MONOCLONAL ABO BLOOD-GROUP SYSTEM HYBRIDOMAS MICE CELL LINE IGM