Avtor/Urednik		Šentjurc, M; Štalc, A; Šuput, D
Naslov		Influence of eqinatoxin II on coronary smooth muscle membrane fluidity
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 431, št. 6 Suppl 2
Leto izdaje		1996
Obseg		str. R317-8
Jezik		eng
Abstrakt		Equinatoxin II forms pores in artificial as well as in natural phospholipid bylayers. As it also reduces the perfusion through the coronary arteries it seemed reasonable to investigate the interaction of this protein with the plasmalemma of the vascular smooth muscle cells more directly with the patch-clamp method and with EPR using the spin probe methyl ester of 5-doxylpalmitate. In the EPR spectra at least three regions in the membranes were identified with different membrane fluidity. Application of 0.1 microM or 1 microM equinatoxin II increases the portion with the lower membrane fluidity, and irreversibly increases the unspecific membrane conductance. This is compatible with the view that equinatoxin II forms pores in the membrane bilayer.
Deskriptorji		MUSCLE, SMOOTH, VASCULAR CNIDARIAN VENOMS MEMBRANE FLUIDITY CORONARY VESSELS PATCH-CLAMP TECHNIQUES RATS ANIMALS, LABORATORY