Avtor/Urednik		Picken, RN; Strle, F; Ružić-Sabljić, E; Maraspin, V; Lotrič-Furlan, S; Cimperman, J; Cheng, Y; Picken, MM
Naslov		Molecular subtyping of Borrelia burgdorferi sensu lato isolates from five patients with solitary lymphocytoma
Tip		članek
Vir		J Invest Dermatol
Vol. in št.		Letnik 108, št. 1
Leto izdaje		1997
Obseg		str. 92-7
Jezik		eng
Abstrakt		Solitary lymphoytoma is a rare coutaneous manifestation of Lyme borreliosis that has been reported almost exclusively from Europe. This suggests that its etiologic agent may be absent or extremely rare on the North American continet. All three species of B.burgdorferi sensu lato knoown to be associated with human Lyme borreliosis (B. burgdorferi sensu stricto, B. garinii, and B. afzelii) have been isolated in Europe, whereas only B. burgdorferi sensu stricto has been founed in North America. This suggests that either B. garinii or B. afzelii might be the etiologic agent of borrelial lymphocytoma. To investigate this hypothesis we characterized five strains of B. burgdorferi sensu lato isolated from lymphocytoma lesions of patients residing in Slovenia. The methods used included: large restriction fragment pattern analysis of restriction enzyme MluI-digested genomic DNA, plasmid profiling, protein profiling, ribotyping using 5S, 16S, and 23S rDNA probes, and polymerase chain reaction amplification of the rrf (5S)-rrl (23S) interaction spacer region. Molecular subtyping showed that four of the five isolates belonged to the species B. afzelii; however, this species is the predominant patient isolate in Slovenia and, therefore, may not represent a preferential association with lymphocytomal The fifth isolate appeared to be most closely related to the DN127 genomic group of organisms. Further characterization of the isolate revealed that it prossessed a unique molecular "fingerprint". The results not only show that borrelial lymphocytoma can be caused by B. afzelii but also demonstrate an association with another genomic group of B. burgdorferi sensu lato that is present in North America as well.
Deskriptorji		BORRELIA BURGDORFERI SKIN NEOPLASMS LYMPHOMA, SMALL LYMPHOCYTIC BIOPSY ANTIGENS, SURFACE DNA PRIMERS DNA, BACTERIAL ELECTROPHORESIS, GEL, PULSED-FIELD ELECTROPHORESIS, POLYACRYLAMIDE GEL GENE AMPLIFICATION LYME DISEASE POLYMERASE CHAIN REACTION RESTRICTION MAPPING SKIN SODIUM DODECYL SULFATE BACTERIAL OUTER MEMBRANE PROTEINS