Avtor/Urednik		Ciccone, E; Pende, D; Viale, O; Ihan, A; Orengo, AM; Biassoni, R; Verdiani, S; Amoroso, A; Moretta, A
Naslov		Involvement of HLA class I alleles in natural killer (NK) cell-specific functions: expression of HLA-Cw3 confers selective protection from lysis by alloreactive NK clones displaying a defined specificity (specificity 2)
Tip		članek
Vir		J Exp Med
Vol. in št.		Letnik 176, št. 4
Leto izdaje		1992
Obseg		str. 963-71
Jezik		eng
Abstrakt		This study was designed to identify the target molecules of the natural killer (NK) cell-mediated recognition of normal allogeneic target cells. As previously shown, the gene(s) governing the first NK-defined allospecificity (specificity 1) were found to be localized in the major histocompatibility complex region between BF gene and HLA-A. In addition, the analysis of a previously described family revealed that a donor (donor 81) was heterozygous for three distinct NK-defined allospecificities (specificities 1, 2, and 5). HLA variants were derived from the B-Epstein-Barr virus cell line of donor 81 by gamma irradiation followed by negative selection using monoclonal antibodies specific for the appropriate HLA allele. Several variants were derived that lacked one or more class I antigen expressions. These variants were analyzed for the susceptibility to lysis by NK clones recognizing different allospecificities. The loss of HLA-A did not modify the phenotype (i.e., "resistance to lysis"). On the other hand, a variant lacking expression of all class I antigens became susceptible to lysis by all alloreactive clones. Variants characterized by the selective loss of class I antigens coded for by the maternal chromosome became susceptible to lysis by anti-2-specific clones. Conversely, variants selectively lacking class I antigens coded for by paternal chromosome became susceptible to lysis by anti-1 and anti-5 clones (but not by anti-2 clones). Since the Cw3 allele was lost in the variant that acquired susceptibility to lysis by anti-2 clones and, in informative families, it was found to cosegregate with the character "resistance to lysis" by anti-2 clones, we analyzed whether Cw3 could represent the element conferring selective resistance to lysis by anti-2 clones.(trunc.)
Deskriptorji		B-LYMPHOCYTES CYTOTOXICITY, IMMUNOLOGIC GENES, MHC CLASS I HLA-C ANTIGENS KILLER CELLS, NATURAL LYMPHOCYTE TRANSFORMATION VARIATION (GENETICS) ALLELES ANTIBODIES, MONOCLONAL BASE SEQUENCE CELL LINE, TRANSFORMED CELLS, CULTURED CLONE CELLS GENE EXPRESSION HAPLOTYPES HERPESVIRUS 4, HUMAN MOLECULAR SEQUENCE DATA OLIGODEOXYRIBONUCLEOTIDES POLYMERASE CHAIN REACTION