Avtor/Urednik		Tomažič, J; Ihan, A
Naslov		Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borbe encephalitis
Tip		članek
Vir		Acta Neurol Scand
Vol. in št.		Letnik 91
Leto izdaje		1996
Obseg		str. 1-5
Jezik		eng
Abstrakt		Introduction. Cerebrospinal fluid (CSF) lymphocytes subsets were examined by flow cytometry in 33 patients with tick-borne encephalitis (TBE) in order to determine their values. Patients and methods. Lymphocytes were isolated from CSF and lymphocyte subsets were determined: lymphocytes T (CD3+), lymphocytes B (CD19+), NK cells (CD3-CD56+), helper T cells (CD3+CD4+) and cytotoxic T cells (CD3+CD8+). The ecpression of IL-2 receptors (CD25+) and transferrin receptors (CD71+) on T cells and HLA-DR molecules on T cell subsets were examined. Furthermore, possible relationships among different YBE patient population variables (gender, age, severity of disease, duration of meningitis) were considered. Results. The analyses of the CSF lymphocyte population subsets are presented. Lymphocytes T (CD3+) were significantly higher in the CSF than in the peripheral blood as was the case with the expression of transferrin receptors (CD71+) on T cells. Lymphocytes B (CD19+) and NK cells (CD3-CD56+) prevailed in the peripheral blood. In the early course of the disease, a higher expression of HLA-DR molecules on T lymphocytes was observed, while later a higher expression of IL-2 receptors (CD25+) was observed. Discussion. Significant differences inlymphocyte subsets between the CSF and the peripheral blood were found. Significant time-dependent changes of CSF lymphocyte subsets during course of infection were observed. The result of the present study give us deeper insight into CNS cellular immunopathogenic mechanisms in patients with TBE.
Deskriptorji		ENCEPHALITIS, TICK-BORNE LYMPHOCYTES FLOW CYTOMETRY CEREBROSPINAL FLUID