Avtor/Urednik		Luzar, Boštjan; Gale, Nina; Kambič, Vinko; Poljak, Mario; Zidar, Nina; Vodovnik, Aleksandar
Naslov		Human papillomavirus infection and expression of p53 and c-erb-2 protein in laryngeal papillomas
Tip		članek
Vir		Acta Otolaryngol Suppl (Stockh)
Vol. in št.		, št. Suppl 527
Leto izdaje		1997
Obseg		str. 120-4
Jezik		eng
Abstrakt		Laryngeal papilloma (LP) is the most frequent benign laryngeal epithelial tumor caused by human papillomaviruses (HPV) types and 11. In the present study, we were interested in whether we can find any prognostic markers which might reflect the biological behavior of the covering epithelium in LP. We focused our attention on the determination of HPV infection, the detection of p53 protein, and c-erbB-2 protein in 24 biopsy specimens of LP. We confirmed the HPV 6 and 11 etiology in 23 of 24 LP. In these lesions the overexpression of p53 protein increased with the grade of epithelial abnormalities.The distribution of positive cells changed from scattered and focal, in simple and abnormal hyperplasia, to diffuse in atypical hyperplasia. It has been shown that in the presence of HPV types 6 and 11 found in LP, p53 can still preserve its tumor suppressor activity. Infection with HPV types 6 and 11 might therefore account for the signifgicantly lower rate of malignant transformation in LP. Two staining patterns for c-erbB-2 protein were observed in the hyperplastic epithelium covering LP: membranous and cytoplasmic. With the increacing grade of epithelial abnormalities, cytoplasmic staining became predominant, and c-erbB-2 positivity sometimes occupied the whole epithelial thickness. This may represent either an alteration in the processing stability of the c-erbB-2 mRNA, gene amplification, or even an artefact.
Deskriptorji		LARYNGEAL NEOPLASMS PAPILLOMA PROTEIN P53 PROTO-ONCOGENE PROTEINS C-ERBB-2 PAPILLOMAVIRUS, HUMAN BIOPSY POLYMERASE CHAIN REACTION HYPERPLASIA