Avtor/Urednik		Seme, Katja
Naslov		Genomska tipizacija izolatov virusa hepatitisa C v Sloveniji
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1997
Obseg		str. 116
Jezik		slo
Abstrakt		Genotyping of hepatitis C virus (HCV) isolates is of particular interest for epidemiological studies. The success of antiviral treatment of chronic HCV infection appears to be related to the viral genotype and the level of viremia. To date, several methods have been described in an attempt to reduce the time-consuming sequencing analysis initially adopted for the determination of HCV genotype. In the present study four different HCV genotyping assays: (i) reverse hybridization with the line probe assay Inno LiPA (Innogenetics, Gent, Belgium) using type specific probes for 5' noncoding region; (ii) restriction fragment length polymorphism analysis of DNA amplified from 5' noncoding region; (iii) amplification of of the core region with genotype-specific primers; and (iv) nested PCR in the core region followed by hybridization to HCV type-specific probes were applied to 40 Slovenian HCV RNA islates in order to determine the concordance between various genotyping systems. The line probe assay was found to be the most appropriate for the routine use and for larger epidemiological studies, due to its acceptable sensitivity, specificity and simplicity. However, the method is hampered occasionally for subtyping by lack of subtype-specific suquence variation in 5' noncoding region. Therefore, all samples in which the line probe assay assigns genotype 2 should be additionally typed by nested PCR in the core region followed by hybridization to HCV type-specific probes. The same should be done for all samples in which the line probe assay assigns genotype 1 but can not clearly differentiate between subtypes. In two Slovene subtype 2c HCV isolates we observed guanine instead of adenine at position -161 of the 5' noncoding region. This position can therefore not be considered as specific for discrimination of subtypes 2a/2c and 2b. To determine the prevalence of HCV genotypes in Slovenia 215 subjects infected with HCV were studied. (Abstract truncated at 2000 charasters).
Deskriptorji		HEPATITIS C HEPATITIS C-LIKE VIRUSES EPIDEMIOLOGY, MOLECULAR GENOTYPE GENOME, VIRAL HEMODIALYSIS HEMOPHILIA SUBSTANCE ABUSE, INTRAVENOUS POLYMERASE CHAIN REACTION IMMUNOBLOTTING OLIGONUCLEOTIDE PROBES BASE SEQUENCE RNA