Avtor/Urednik		Saunders, PTK; Millar, MR; Gaugan, J; Kinnell, CMc; Majdič, G; Sharpe, RM
Naslov		Gene expression during spermatogenesis: regulation of protein expression by testosterone
Tip		članek
Vir		In: Cestnik V, Pogačnik A, editors. Referati 6. Zavrnikovega spominskega sestanka; 1995 nov 9-11; Lipica. Ljubljana: Veterinarska fakulteta,
Leto izdaje		1997
Obseg		str. 3-7
Jezik		eng
Abstrakt		Testosterone is essential for maintenance of spermatogenesis. We have shown that in the rat, the pattern of secretion of proteins by seminiferous tubules is stage dependent with highest levels at stage VI-VIa of sparmatogenic cycle. Testosterone withdrawal results in abolition of the increase in protein secretion at these stages. Androgen action in target tissues is mediated via a specific intracellular receptor. Using immunocytochemical localisation following microwave antigen retrieval, we have studied the pattern of expression of androgen receptor (AR) in the testis. Peritabular cells contained abundant nuclear AR during fetal and neonatal life. However as germ cells developed within the seminiferous tubules immunostaining in Sertoli cell nuclei increased and began to vary between tubules depending upon their germ cell complememnt. In the Sertoli cells of the adult rat expression of AR was stage dependent with uncreasing levels of nuclear immunostaining from stage II to stage VII and very low levels at other stages. Immunostaining in all cells was reduced following androgen withdrawal. One explanation for the stage dependent affects of testosterone in the rat testis may therefore be the pattern of expression of the AR in this species.
Deskriptorji		SPERMATOGENESIS TESTOSTERONE RECEPTORS, ANDROGEN RATS