Avtor/Urednik		Majdič, G; Sharpe, RM; O'Shaughnessy, PJ; Saunders, PTK
Naslov		Oestrogen effects on testicular expression of 17 alpha hydroxylase
Tip		članek
Vir		In: Cestnik V, Pogačnik A, editors. Referati 6. Zavrnikovega spominskega sestanka; 1995 nov 9-11; Lipica. Ljubljana: Veterinarska fakulteta,
Leto izdaje		1997
Obseg		str. 9-14
Jezik		eng
Abstrakt		Testosterone is required for normal development of the male reproductive tract. Synthesis of testosterone occurs in the Leydig cells and is dependent upon the expression of enzymes including cytochrome P450 17alpha-hydroxylase/C17-20 lyase (P450cl7). We have investigated whether maternal exposure to oestrogenic chemicals was able to affect Leydig cell function in the developing male fetus. Pregnant rats were injected subcutaneously with diethylstilbestrol (DES), 4-octylphenol (OP) or vehicle (oil, control) on days 11.5 and 15.5 post coitum and fetal testes recovered on day 17.5 of gestation.There was no difference between fetuses from control and treated mothers in the number of Leydig cells as determined by immunocytochemistry with an antibody directed against 3beta-hydroxysteroid dehydrogenase. However, there was a reduction in the amount of P450c17 detected by immunocytochemistry or by measurement of enzymatic activity in testes from the groups given DES or OP. In situ hybridisation revealed that these changes were mirrored by similar reductions in P450c17 mRNA. These results provide a mechanism by which inappropriate exposure of the fetus to oestrogenic chemicals might have an adverse affect on fetal steroid synthesis and masculinization.
Deskriptorji		ESTROGENS STEROID 17 ALPHA-MONOOXYGENASE RATS