Avtor/Urednik		Cimerman, Nina; Drobnič-Košorok, Marinka; Korant, Bruce D; Turk, Boris; Turk, Vito
Naslov		Characterization of cystatin C from bovine parotid glands: cysteine proteinase inhibition and antiviral properties
Tip		članek
Vir		Biol Chem Hoppe Seyler
Vol. in št.		Letnik 377, št. 1
Leto izdaje		1996
Obseg		str. 19-23
Jezik		eng
Abstrakt		Cystatin C, a low Mr cysteine proteinase inhibitor was isolated from bovine parotid glands by a procedure which includes alkaline treatment of the homogenate, affinity chromatography, gel filtration and ion exchange chromatography. The purified inhibitor has a pl of 8.0 and Mr of 14500. The identity with bovine cystatin C from colostrum was confirmed by N-terminal sequence of the inhibitor and amino acid composition. Cystatin C rapidly (kass = 5.5 x 10(7) M-1s-1) and tightly inhibits papain (Ki = 0.02 nM), whereas its interaction with bovine cathepsin B is substantially weaker (Ki = 4.4 nM). Bovine cystatin C also shows a weak antiviral effect on poliovirus infected human Hela cells.
Deskriptorji		ANTIVIRAL AGENTS CYSTATINS CYSTEINE PROTEINASE INHIBITORS PAROTID GLAND AMINO ACID SEQUENCE CATTLE CYTOPATHOGENIC EFFECT, VIRAL KINETICS MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT PLAQUE ASSAY POLIOVIRUSES