Avtor/Urednik     Cimerman, Nina; Drobnič-Košorok, Marinka; Korant, Bruce D; Turk, Boris; Turk, Vito
Naslov     Characterization of cystatin C from bovine parotid glands: cysteine proteinase inhibition and antiviral properties
Tip     članek
Vir     Biol Chem Hoppe Seyler
Vol. in št.     Letnik 377, št. 1
Leto izdaje     1996
Obseg     str. 19-23
ISSN     0177-3593
Jezik     eng
Abstrakt     Cystatin C, a low Mr cysteine proteinase inhibitor was isolated from bovine parotid glands by a procedure which includes alkaline treatment of the homogenate, affinity chromatography, gel filtration and ion exchange chromatography. The purified inhibitor has a pl of 8.0 and Mr of 14500. The identity with bovine cystatin C from colostrum was confirmed by N-terminal sequence of the inhibitor and amino acid composition. Cystatin C rapidly (kass = 5.5 x 10(7) M-1s-1) and tightly inhibits papain (Ki = 0.02 nM), whereas its interaction with bovine cathepsin B is substantially weaker (Ki = 4.4 nM). Bovine cystatin C also shows a weak antiviral effect on poliovirus infected human Hela cells.
Deskriptorji     ANTIVIRAL AGENTS
CYSTATINS
CYSTEINE PROTEINASE INHIBITORS
PAROTID GLAND
AMINO ACID SEQUENCE
CATTLE
CYTOPATHOGENIC EFFECT, VIRAL
KINETICS
MOLECULAR SEQUENCE DATA
MOLECULAR WEIGHT
PLAQUE ASSAY
POLIOVIRUSES