Avtor/Urednik		Dolenc, Iztok; Turk, Boris; Kos, Janko; Turk, Vito
Naslov		Interaction of human cathepsin C with chicken cystatin
Tip		članek
Vir		FEBS Lett
Vol. in št.		Letnik 392, št. 3
Leto izdaje		1996
Obseg		str. 277-80
Jezik		eng
Abstrakt		Cathepsin C was purified from human spleen by a rapid procedure, which included homogenization, ammonium sulfate precipitation, gel filtration on Sephracryl S-200 and finally affinity chromatography on chicken cystatin-Sepharose. The interaction between cathepsin C and chicken cystatin was further characterized. It was found to be accompanied by a maximum decrease in fluorescence emission intensity at 336 nm. Fluorescence titration showed that human cathepsin C can bind four chicken cystatin molecules. The 4:1 binding stoichiometry was confirmed by titration monitored by the loss of enzyme activity. A non-competitive-competitive type of inhibition was determined from a double-reciprocal Lineweaver-Burk plot with a Ki value of 0.22 nM for the non-competitive inhibition.
Deskriptorji		CHROMATOGRAPHY, AFFINITY CYSTATINS DIPEPTIDYL PEPTIDASES CHICKENS SPECTROMETRY, FLUORESCENCE TITRIMETRY