Avtor/Urednik		Tomažič, J; Ihan, A
Naslov		Flow cytometric analysis of lymphocytes in cerebrospinal fluid in patients with tick-borne encephalitis
Tip		članek
Vir		Acta Neurol Scand
Vol. in št.		Letnik 95, št. 1
Leto izdaje		1997
Obseg		str. 29-33
Jezik		eng
Abstrakt		INTRODUCTION: Cerebrospinal fluid (CSF) lymphocyte subsets were examined by flow cytometry in 33 patients with tick-borne encephalitis (TBE) in order to determine their values. PATIENTS AND METHODS: Lymphocytes were isolated from CSF and lymphocyte subsets were determined: lymphocytes T (CD3+), lymphocytes B (CD19+), NK cells (CD3-CD56+), helper T cells (CD3+CD4+) and cytotoxic T cells (CD3+CD8+). The expression of IL-2 receptors (CD25+) and transferrin receptors (CD71+) on T cells and HLA-DR molecules on T cell subsets was examined. Furthermore, possible relationships among different TBE patient population variables (gender, age, severity of disease, duration of meningitis) were considered. RESULTS: The analyses of the CSF lymphocyte population subsets are presented. Lymphocytes T (CD3+) were significantly higher in the CSF than in the peripheral blood as was the case with the T cells that expressed transferrin receptors (CD71). Lymphocytes B (CD19+) and NK cells (CD3-CD56+) prevailed in the peripheral blood. In the early course of the disease, a higher expression of HLA-DR molecules on T lymphocytes was observed, while later a higher expression of IL-2 receptors (CD25+) was observed. DISCUSSION: Significant differences in lymphocyte subsets between the CSF and the peripheral blood were found. Significant time-dependent changes of CSF lymphocyte subsets during course of infection were observed. The results of the present study give us deeper insight into CNS cellular immunopathogenic mechanisms in patients with TBE.
Deskriptorji		CELL SEPARATION CEREBROSPINAL FLUID ENCEPHALITIS, TICK-BORNE FLOW CYTOMETRY T-LYMPHOCYTES ADULT ANTIBODIES, MONOCLONAL B-LYMPHOCYTES ENZYME-LINKED IMMUNOSORBENT ASSAY HLA-DR ANTIGENS IGG IGM KILLER CELLS, NATURAL