Avtor/Urednik		Popović, Mara
Naslov		Spreminjanje antigenskih značilnosti Schwannovih celic v ishiadičnem živcu podgane po poškodbi
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1996
Obseg		str. 44
Jezik		slo
Abstrakt		In contrast to the central nervous system, peripheral nerves display remarkable ability to regenerate after injury which is due to favourable axonal microenvironment in a peripheral nerve. Its components, i.e. the Schwann cells (SC) and their extracellular matrix, support axonal regeneration. We created three experimental models of the sciatic nerve injury in the rat with appropriate controls in order to study the antigenic properties of SC into the absence of axons, their ability to migrate, and their possible influence on other non-neural cells in the peripheral nerve. In addition, the effect of axons on these properties of SC, either after delayed axonal contact with migrating SC or when the SC were migrating together with the regenerating axons, will be investigated. Migration of SC into the acellular distal nerve segment without interrupting the continuity of their basal lamina tubes was achieved by fireezing the distal nerve stump. This procedure destroyed the cells but left the extracellular matrix in the nerve virtually intact. SC proliferating in situ during Wallerian degeneration, either in presence or in the absence of regenerating axons, and SC in the normal nerve served as controls. We assumed a) that SC are able to migrate well even in the absence of axons provided their basal lamina tubes remained preserved, b) that the migrating SC dedifferentiate more that the SC proliferating in situ in absence of axons, and c) that other migrating cells invading the acellular distal nerve segment together with SC can reconstruct the destroyed perineurium in the previously frozen nerve segment. We further hypothesized that all these processes should be strongly influenced by the regenerating axons. (Abstract truncated at 2000 characters).
Deskriptorji		SCIATIC NERVE NERVE REGENERATION SCHWANN CELLS RATS IMMUNOHISTOCHEMISTRY MICROSCOPY, ELECTRON RECEPTORS, NERVE GROWTH FACTOR NERVE TISSUE PROTEIN S 100