Avtor/Urednik		Žel, Jana; Debeljak, Nika; Ucman, Robert; Ravnikar, Maja
Naslov		The effect of jasmonic acid, sucrose and darkness on garlic (Allium sativum L. ev. Ptujski jesenski) bulb formation in vitro
Tip		članek
Vir		In Vitro Cell Dev Biol, Plant
Vol. in št.		Letnik 33
Leto izdaje		1997
Obseg		str. 231-5
Jezik		eng
Abstrakt		The effect of sucrose, jasmonic acid (JA) and darkness on bulb formation of garlic Allium sativum L. ev. Ptujski jesenski was studied in vitro. B5 medium supplemented with 3% sucrose, 6 microM JA and 5 microM 2-isopentenyl adenine (2iP) was used for shoot induction on garlic basal plates. For bulb induction, explants with developed shoots were transferred onto media with 3% or 8% sucrose in the presence or absence of 5 microM JA. Sucrose (8%) significantly increased the percentage of shoots which formed bulbs by 86-90%, bulb diameter and the number of bulbs per basal plate. On medium supplemented with JA, the average number of bulbs per basal plate was 11.5. Growth of explants in the dark was ineffective for stimulating bulb formation. Simultaneous use of JA and sucrose can improve garlic micropropagation via bulb formation, without intermediate callus formation.
Deskriptorji		GARLIC TISSUE CULTURE SUCROSE DARKNESS PLANT SHOOTS PLANT GROWTH REGULATORS ISOPENTENYLADENOSINE PLANT ROOTS