Avtor/Urednik		Elgh, Fredrik; Lundkvist, Ake; Alexeyev, Oleg A; Stenlund, Hans; Avšič-Županc, Tatjana; Hjelle, Brian; Wang-Lee, Ho; Smith, Kenneth J; Vainiopaa, Raija; Wiger, Donna; Wadell, Goran; Juto, Per
Naslov		Serological diagnosis of hantavirus infections by an enzyme-linked immunosorbent assay based on detection of immunoglobulin G and M responses to recombinant nucleocapsid proteins of five viral serotypes
Tip		članek
Vir		J Clin Microbiol
Vol. in št.		Letnik 35, št. 5
Leto izdaje		1997
Obseg		str. 1122-30
Jezik		eng
Abstrakt		Worldwide, hantaviruses cause more than 100,000 human infections annually. Rapid and accurate methods are important both in monitoring acute infections and for epidemiological studies. We and others have shown that the amino temrini of hantavirus nucleocapsid proteins (Ns) are sensitive tools for the detection of specific antibodies in hantavirus disease. Accordingly, we expressed truncated Ns(amino acids 1 to 117) in Escherichia colli from the five hantaviruses known to be pathogenic to man; Hantaan (HTN), Seoul (SEO), Dobrava (DOB), Sin Noombre (SN), and Puumala (PUUM) viruses. In order to obtain pure antigens for use in an enzyme-linked immunosorbent assay (ELISA), the recombinant proteins were purified by polyhistidine-mental chelate affinity chromatography. Polyclonal animal antisera and a panel of serum speciemns from hantavirus-infected individuals from Scandinavia, Slovenia, Russia, Korea, China, and the United States were used to evaluate the usefulness of the method. With both human and animal sera, it was possible to designate the antibody response into two groups: those with HTN, SEO, and DOB virus reactivity on the one hand and those with SN and PUU virus reactivity on the other. In sera from Scandinavia, European Russia, and the United States, the antibody response was directed mainly to the PUU and SN virus group. The sera from Asia reacted aknist exckysuvekt wutg tge HTN, SEO, and DOB types of viruses. This was true for both the immunoglobulin M (IgM) and IgG antibody responses, indicating that this type of discrimination can be done during the acute phase of hantavirus infections. Both the HTN, SEO, and DOB virus and the PUU and SN virus types of antibody response patterns were found in patients from the Balkan region (Slovenia).
Deskriptorji		HANTAVIRUS INFECTIONS HANTAVIRUS CAPSID IGG IGM ENZYME-LINKED IMMUNOSORBENT ASSAY RABBITS RECOMBINANT PROTEINS SERODIAGNOSIS