Avtor/Urednik		Vitas, Marko; Pajič, Tadej; Kelly, Steven L; Komel, Radovan
Naslov		11beta-hydroxysteroid dehydrogenase activity in progesterone biotransformation by the filamentous fungus Cochliobolus lunatus
Tip		članek
Vir		J Steroid Biochem Mol Biol
Vol. in št.		Letnik 63, št. 4-6
Leto izdaje		1997
Obseg		str. 345-50
Jezik		eng
Abstrakt		Progesterone biotransformation was examined in relation to hydroxylating and dehydrogenating enzymes of Cochliobolus lunatus. 11beta-hydroxysteroid dehydrogenase activity (11beta-HSD) was located in cytosolic fraction and was NADP-dependent, inducible by progesterone and apparently unidirectional. Several inhibitors of 11beta-hydroxysteroid dehydrogenase were tested; furosemide, glycyrrhizic-acid and carbenoxolone did not influence the dehydrogenation of 11beta-hydroxy-4-pregnene-3,20-dione to 4-pregnene-3,11,20-trione, although grapefruit juice significantly reduced the rate of progesterone hydroxylation.
Deskriptorji		ASCOMYCETES BIOTRANSFORMATION PROGESTERONE HYDROXYSTEROID DEHYDROGENASES DENSITOMETRY SPECTRUM ANALYSIS, MASS MICROSOMES CYTOSOL