Avtor/Urednik		Pungerčar, Jože; Anderluh, Gregor; Maček, Peter; Gubenšek, Franc; Štrukelj, Borut
Naslov		Sequence analysis of the cDNA encoding the precursor of equinatoxin V, a newly discovered hemolysin from the sea anemone Actinia equina
Tip		članek
Vir		Biochim Biophys Acta
Vol. in št.		Letnik 1341
Leto izdaje		1997
Obseg		str. 105-7
Jezik		eng
Abstrakt		A cDNA encoding the 214-amino-acid (aa) precursor of equinatoxin V (EqtV) has been isolated from an Actinia equina cDNA library. The sequence of the mature toxin is preceded, as that of EqtII, by a signal peptide of 19 aa and a hydrophilic propeptide of 16 aa ending with a pair of basic residues. This is similar to the precursors of calitoxins from another sea anemone Calliactis parasitica and to those of some antimicrobial peptides of the magainin and dermaseptin families from vertebrates. The deduced aa sequence of the potential cell atachment Arg-Gly-Asp motif-containing EqtV shows 82% identity to that of EqtII.
Deskriptorji		CNIDARIAN VENOMS HEMOLYSINS PROTEIN PRECURSORS SEA ANEMONES DNA, COMPLEMENTARY AMINO ACID SEQUENCE BASE SEQUENCE MOLECULAR SEQUENCE DATA SEQUENCE ALIGNMENT SEQUENCE ANALYSIS, DNA SIGNAL PEPTIDES CLONING, MOLECULAR BLOTTING, WESTERN ELECTROPHORESIS, POLYACRYLAMIDE GEL