Avtor/Urednik		Snoj-Cvetko, Erika
Naslov		Izooblike težkih verig miozina v regeneratih mišic extensor digitorum longus in soleus podgane
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1995
Obseg		str. 64
Jezik		slo
Abstrakt		Expression of myosin heavy chain (MHC) isoforms in adult slow and fast muscles can be modified by changing their stimulation pattern or after crossinnervation, but only within a certain adaptive range. To test adaptive range conveyed by the satellite cells within the two muscle types, rat soleus (SOL) and extensor digitorum longus (EDL) muscles were injured by bupivacain (Marcaine) and allowed to regenerate either as autotransplant (innervated by its appropriate nerve) or heterotransplant, being cross-transplanted to the site of the other muscle (cross-innervated). Three months later, muscle samples were proceeded for histochemical and immunohistochemical analysis of fibre types and MHC isoforms. Additionally, SDS gel electrophoresis and in situ hybridization techniques were ued for determining MHC protein isoforms and mRNA transcripts. SOL and EDL muscles, regenerating in their own beds, became histochemically similar to the control muscles. Only MHC-1 and MHC-2A were observed in SOL transplants. In the EDL transplants, about 57% of fibres contained only one MHC isoform (MHC-2B, MHC-2A, MHC-2X/D, MHC-1), all the rest were hybrid fibres, containing two or three MHCs. SOL muscle regenerating in the place of EDL expressed all MHCs, including MHC-2B. However, compared to the homotopic EDL transplant, the pecentage of fibres containing MHC-1 (either pure or hybrid) was higher. EDL muscle regenerating in the place of SOL muscle expressed MHCs similar to the SOL muscle and to homotopic SOL transplants (preponderance of MHC-1, about 7% of MHC-2A and about 6% of hybrid fibres coexpressing MHC-1 and MHC-2A). The adaptive range of regenerating muscles is evidently much than it is in adult muscles.(Abstract truncated at 2000 characters.)
Deskriptorji		MUSCLE, SKELETAL MYOSIN HEAVY CHAINS MYOSIN LIGHT CHAINS RATS TRANSPLANTATION, AUTOLOGOUS TRANSPLANTATION, HOMOLOGOUS RNA, MESSENGER HISTOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY MYOFIBRILS