Avtor/Urednik		Bresjanac, M; Popović, M
Naslov		In vivo study of regeneration of Schwann cell-deprived axons and migration of denervated Schwann cells
Tip		članek
Vir		In: Stalberg EV, de Weerd AW, Zidar J, editors. ECCN 98. 9th European congress of clinical neurophysiology; 1998 Jun 4-7; Ljubljana. Bologna: Monduzzi Editore,
Leto izdaje		1998
Obseg		str. 39-42
Jezik		eng
Abstrakt		It is widely accepted that the normal function and successful regeneration of the mammalian peripheral nerve hinges upon the trophic and guiding influences of the peripheral glia, the Schwann cells (SC). However, ample evidence points to the reciprocal nature of the axon-SC relation: SC seem to depend on axonal contact for their normal phenotype and function. Following a peripheral nerve injury, both neurons and SC undergo dramatic changes and many of those are believed to aid the regeneration process, but their roles are insufficiently understood. In order to further elucidate the mechanisms of peripheral nerve regeneration in vivo, we designed an experimental model to study separately (1) axonal regeneration in the absence of SC support, and (2) the response of axon-deprived ("denervated") SC to nerve injury. In the first series of experiments, a long segment of the rat sciatic nerve just distal to a crush site was rendered acellular, while the proliferation of SC in the proximal stump and their migration alongside the growing axons was inhibited by mitomycin C injection into the proximal nerve segment. The effects of mitomycin C on SC were determined by immunohistochemistry (IHC) to proliferating nuclear antigen (PCNA) and S100 protein on adjacent nerve sections. Axonal elongation through he acellular distal nerve segment was tested by a nerve pinch test at 6 days post-crush as well as by IHC to neurofilament (NF). Transverse sections of the nerve were also studied by electron microscopy (EMi). Long-term effects of cell support deprivation on axon regeneration were studied by the same means at 3, 4 and 8 weeks post-crush. In the second experiment, SC population above the nerve crush wa denervated by a more proximal nerve cut, and allowed to proliferate and repopulate an acellular segment distally to the crush.(Abstract truncated at 2000 characters.)
Deskriptorji		NERVE REGENERATION AXONS SCHWANN CELLS RATS, WISTAR NERVE CRUSH