Avtor/Urednik		Brank, Martina
Naslov		Uravnavanje sinteze skeletnomišične acetilholinesteraze na ravni poliribosomov
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1998
Obseg		str. 84
Jezik		slo
Abstrakt		Elucidation of the mechanisms modulating the expression of various genes in mammalian organisms is the basic prerequisite for understanding the differentiation, regeneration, aging and plasticity of the tissues. Hence, the major part of the fundamental biomedical research nowadays focuses on investigation of these mechanisms. Intracellular modulation of the expression of specific genes may take place on several levels, from transcription to translational and posttranslational processes. In the first part of the present study, we examined the applicability of in vitro models for the investigations of the regulation of acetylcholinesterase (AChE; E.C. 3.1.1.&) synthesis at the level of AChE mRNA stability. Degradatio of the in vitro transcribed acetylcholinesterase mRNA (Ache mRNA) was analyzed by High Performance Liquid Chromatography (HPLC), a method not tested yet in such sutdies. In the second part of our thesis, we examined the possibility of stuying AChE synthesis at the translational and early posttranslational stages by measuring AChE activity in the specially prepared polysomal fraction. This approach has also not been described before. In the third part of our thesis we tested this new approach by studying AChE synthesis under the conditions of glucocorticoid myopathy (G.M.). Namely, it has been suggested that under these conditions, protein synthesis in the skeletal muscle is affected at the translational level. The following three hypotheses were tested: 1. Rat polysomal musculoskeletal fraction contains specific RNase activity. 2. AChE activity measured in the polysomal musculoskeletal fraction reflects the earliest forms of catalytically active enzyme. 3. A decrease in AChE activity observed under the conditions of G.M. results from impairment of AChE synthesis at the translational and/or early posttranslational level.(Abstract truncated at 2000 characters.)
Deskriptorji		MUSCLE, SKELETAL ACETYLCHOLINESTERASE POLYRIBOSOMES RATS RNA, MESSENGER TRANSLATION, GENETIC CHROMATOGRAPHY, HIGH PRESSURE LIQUID