Avtor/Urednik		Ocepek, M; Zabavnik-Piano, Jelka; Posedi, J; Pislak, Metka
Naslov		Molekularnobiološka tipizacija mikobakterij iz sklopa Mycobacterium avium, izoliranih pri domačih živalih
Prevedeni naslov		Molecular biology typisation of Mycobacterium avium complex isolated from domestic animals
Tip		članek
Vir		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Leto izdaje		1998
Obseg		str. 121-4
Jezik		slo
Abstrakt		Mycobacteria of M. avium complex were typed using molecular biology techniques of polymerase chain reaction (PCR) and restriction fragment lenght polymorphism (RELP) which enabled us to detect the differences in the structure of the genetic material. Genomic material of 120 isolates of mycobacteria of M. avium complex obtained from domestic animals were tested fot the presence of the specific insertion sequence IS901 and flanking region FR300 by PCR reaction. The fragment IS901 was detected in 45 isolates (37.5%), two from these were isolated from chicken, one was from cattle, one from cockatoo, one from crane and forty isolates from pigs. Fragment FR300 was detected in 54 isolates (45.0%), one isoalte was from dog and 53 isolates from pigs. In 21 isolates (17.5%) no fragments were detected. In addition, RFLP analysis was performed in 50 isolates were IS901 was detected. The obtained resultas indicate that in Slovenia at least seven RELP types of mycobacteria from the M. avium complex are prsent in domestic animals
Deskriptorji		MYCOBACTERIUM AVIUM COMPLEX SEROTYPING ANIMALS, DOMESTIC