Avtor/Urednik		Prinčič, Alenka; Mahne, Ivan; Megušar, France; Laanbroek, Hendrikus J
Naslov		Strukturne spremembe nitrifikacijskih združb pri selektivnih dejavnikih okolja: DGGE (denaturation gradient gel electrophoresis) in filogenetska analiza
Prevedeni naslov		Response of nitrifying communities to selective environmental conditions: DGGE (denaturation gradient gel electrophoresis) and phylogenetic analysis
Tip		članek
Vir		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Leto izdaje		1998
Obseg		str. 217-20
Jezik		slo
Abstrakt		Changes in nitrifying community structure in response to different prevailing ammonium concentrations (50, 500, 1000, 3000 mg/l), pH values (6.0, 7.0, 8.2) and oxygen levels (1%, 7%, 21%) were studied in experimental reactors containing bacterial community from a waste water. Denaturing gradient del electrophoresis (DGGE) of PCR products obtained using ammonia oxidizer specific rDNA primers revealed large community structure shift induced by high ammonium levels and was reversible once the ammonium was returned to its original low level. The changes in community structure brought about by both pH extremes, however were irreversible. Ribosomal DNA sequence analysis revealed that high ammonium and deviating pH environments stimulated growth of ammonia oxidizer strains that are substantially different from their nearest relatives, Nitrosomonas eutropha C-91 in Nitrosomonas europaea.
Deskriptorji		NITROSOMONAS NITROSATION AMMONIUM COMPOUNDS EVOLUTION, MOLECULAR ELECTROPHORESIS HYDROGEN-ION CONCENTRATION DNA, RIBOSOMAL OXYGEN