Avtor/Urednik		Ihan, A
Naslov		Aktivacija sluzničnih limfocitov T ob okužbi s H. pylori
Prevedeni naslov		Gastric mucosal T lymphocyte activation upon H. pylori infection
Tip		članek
Vir		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Leto izdaje		1998
Obseg		str. 433-4
Jezik		slo
Abstrakt		Helicobacter pylori infects an estimated 50% of the world population, however only a small proportion, however only a small proportion of individuals develop clinical symptoms of gastric, peptic ulceration or gastric cancer. The variations in disease presentation may be due to differences in bacterial virulence and/or immune response to the pathogen. In the previous study we reported an increased expression of the IL-2 receptor in duodenal ulcer (DU) patients. Present study examined the expression of IL-2 receptor and intracellular lymphokine production in gastric mucosa infiltrating T lymphocytes in DU patients before and after H. pylori eradication. T lymphocytes were isolated from gastric mucosa biopsies by using mechanical and enzymatic tissue desegregation. Ficoll-purified lymphocytes were incubated with monoclonal antibodies and analysed by using 4-colour cytometry analysis for the IL-2 receptor (CD25) and intracellular interferon gamma (IFNgamma) and IL-4 expression. Lymphocytes from 24 Helicobacter pylori infected patients with severe gastric mucosa infiltration (g2 and G3 histological type in Sydney classification) were analysed. We demonstrated a significant decrease in IL-2 receptor expression on gastric mucosa T cells 3 and 12 months after eradication of H. pylori. We also demonstrated a diminished interferon gamma production 3 and 12 months after H. pylori eradication. Our results suggest that cellular immune activation in gastric mucosa is reversibly dependent on the presence of H. pylori.