Avtor/Urednik     Maček-Lebar, A; Kopitar, NA; Ihan, A; Serša, G; Miklavčič, D
Naslov     Significance of treatment energy in cell electropermeabilization
Tip     članek
Vir     Electro Magnetobiol
Vol. in št.     Letnik 17, št. 2
Leto izdaje     1998
Obseg     str. 255-62
Jezik     eng
Abstrakt     The effect of applied electrical energy on cell survival and permeabilization of the plasma membrane under in vitro conditions were examined. Cell viability was measured with a colony-forming assay, permeabilization of the plasma membrane by means of propidium iodide (PI) uptake. Cells were exposed to five regimens of electrical treatment parameters. For a given voltage, applied electrical energy was the same for all regimens. In all applied regimes, cell survival was comparable. The differences in PI uptake between applied regimens were significant. The highest percentage of stained living cells (76%) was observed with four long pulses (200 micro s) with a repetition frequency of 0.5 Hz at 200 V. At regimens with higher numbers of shorter pulses, the percentage of stained living cells was considerably lower. According to our results, total applied electrical energy is not a determinant parameter for drug uptake and cell survival under in vitro conditions.
Deskriptorji     CELL LINE, TRANSFORMED
CELL SURVIVAL
ELECTROPORATION
HAMSTERS
PROPIDIUM
CELL MEMBRANE PERMEABILITY