Avtor/Urednik		Črešnar, Bronislava; Plaper, Andreja; Breskvar, Katja; Hudnik-Plevnik, Tamara
Naslov		cDNA sequence and deduced amino acid sequence of a fungal stress protein induced in Rhizopus nigricans by steroids
Tip		članek
Vir		Biochem Biophys Res Commun
Vol. in št.		Letnik 250, št. 3
Leto izdaje		1998
Obseg		str. 664-7
Jezik		eng
Abstrakt		cDNA clone was isolated from lambda gt11 library prepared from Rhizopus nigricans after growing the fungus in the presence of progesterone. Northern blot analysis of total RNA showed that expression of corresponding mRNA was up-regulated in R. nigricans after treatment with different steroids and after exposure of the fungus to heat shock or osmotic stress. Sequence analysis revealed an open reading frame for a 364-amino-acid polypeptide. The predicted amino acid sequence exhibited significant similarity to several sugar epimerases in two domains common to these enzymes. Our results suggest that the analyzed cDNA is coding for a fungal stress inducible protein beloging to sugar epimerases.
Deskriptorji		RHIZOPUS STEROIDS SEQUENCE ANALYSIS, DNA DNA, COMPLEMENTARY AMINO ACID SEQUENCE PROGESTERONE DESOXYCORTICOSTERONE TESTOSTERONE RNA BLOTTING, NORTHERN