Avtor/Urednik		Simčič, Saša
Naslov		Klinični pomen dokazovanja sestavin naravne odpornosti - fagocitoza
Prevedeni naslov		The clinical importance of evaluation of the phagocytic function as a part of the innate immunity
Tip		članek
Vir		Med Razgl
Vol. in št.		Letnik 37, št. Suppl 5
Leto izdaje		1998
Obseg		str. 101-7
Jezik		slo
Abstrakt		The phagocytic response involves the receptor-mediated binding and internalisation of invading pathogens, the activation of a NADPH-oxidase catalysed oxidative burst and the release of microbicidal and degradative granular proteins. neutrophils from patients with X-linked chronic granulomatous disease fail to produce a significant oxidative burst following stimulation. We have used phorbol myristate acetate and apsonised zymosan A as inducers of luminol-enhanced chemiluminescence as a routine screening for deficiences of the neutrohil oxidative burst and serum opsonic activity. neutrophils from six patients with X-linked chronic granulomatous disease produced no peak chemiluminescence with respect to healthy individuals; three of them had normal, and one patient had reduced serum opsonic activity.When serum samples of three chronic granulomatous disease patients were tested during a period of nonsystemic fungal infection, significantly elevated levels of myeloperoxidase and lactoferrin determined by ELISA were found. The chronic granulomatous disease patient without infection had normal serum levels of granular proteins. Our data suggest that this model seems to be appropriate for detecting phagocytic function deficiencies.
Izvleček		Fagocitoza obsega preko receptorja posredovano vezavo povzročiteljev bolezni s fagociti in njihovo zajetje v fagosom, temu pa sledi z NADPH-oksidazo kataliziran oksidativni izbruh in sproščanje mikrobicidnih proteinov in razgradnih encimov iz ganul fagocitov. Nevtrofilci bolnikov s kronično granulomatozno boleznijo, na X-kromosom vezavo recesivno obliko, se na spodbujanje z različnimi agonisti ne odzovejo z oksidativnim izbruhom. V tej študiji smo za ugotavljanje pomanjkljivosti pri oksidativnem izbruhu in serumski opsonizacijski dejavnosti uporabili forbolov miristat acetat in posoniziran zymosan A, oksidativni odziv pa merili z luminolom ojačano kemiluminiscenco. nevtrofilci Šestih bolnikov s kronično granulomatozno boleznijo se v primerjavi z nevtrofilci zdravih dajalcev na spodbujenje s forbolovim miristat acetatom niso odzvali s kemiluminiscenco. Pri treh bolnikih smo izmerili normalno serumsko opsonizacijsko sposobnost, pri enem bolniku pa je bila opsonizacija zmanjšana. Trije bolniki s kronično granulomatozno boleznijo, ki so prebolevali nesistemske glivične okužbe, so imeli povišano serumsko koncentracijo mieloperoksidaze inlaktoferina, ki smo ju določili z ELISA. Bolnik, v obdobju brez okužbe, je imel normalne serumske vrednosti obeh granularnih proteinov. Naši rezultati potrjujejo uporabnost tega modela za ugotavljanje pomanjkljivosti v delovanju fagocitov.
Deskriptorji		PHAGOCYTOSIS GRANULOMATOUS DISEASE, CHRONIC IMMUNITY, NATURAL CHEMILUMINESCENCE NEUTROPHIL ACTIVATION TETRADECANOYLPHORBOL ACETATE OPSONINS