Avtor/Urednik     Debeljak, Maruša
Naslov     Molekularna karakterizacija promotorja in 3'-neprevedene regije gena za kapa-kazein pri govedu
Prevedeni naslov     Molecular characterisation of promoter and 3'utr of the bovine kappa-casein gene
Tip     monografija
Kraj izdaje     Ljubljana
Založnik     Medicinska fakulteta
Leto izdaje     1998
Obseg     str. 101
Jezik     slo
Abstrakt     Differential production and expression between allelic variants A and B of bovine kappa-casein (kappa-CN) has been observed at the protein and mRNA levels in previous studies. This differences in allelic expression have an important effect on the casein composition of milk. In this investigation, several possible molecular effects were studied to understand the nature of this phenomena. Binding regions for three different transcription factors to a 450-bp fragment of the proximal promoter region of the bovine kappa-CN gene were detected using footprinting and mobility shift analysis. Binding sites for NF1 (nt-342 to nt-357), AP2 (nt-411 to nt-422), and possibly, MGF (nt-380 to nt-393) were identified in the sequence. We have not identified any genetic polymorphisms in the proximal promoter region (1073nt) of the kappa-CN gene in Holstein-Friesian cattle having the milk protein genotype AA, BB and EE. Therefore, we concluded that differential allelic expression is not due to changes of transcriptional activity of the proximal kappa-CN promoter. Several point mutation were detected in the 3' flanking region of the bovine kappa-CN gene between allelic variants A and B. It is possible that these mutations may play a role in stability and translation of the mRNA. This has not been confirmed, however we found the RNA transcripts between the two allelic variants to be different in length, suggesting the possibility that the part of the differential allelic expression could be due to posttranscriptional regulation.
Deskriptorji     CASEINS
POINT MUTATION
MILK
TRANSCRIPTION, GENETIC
TRANSCRIPTION FACTORS
CATTLE
ALLELES
PROMOTER REGIONS (GENETICS)
GENOTYPE
DNA FOOTPRINTING
POLYMERASE CHAIN REACTION
DNA
ELECTROPHORESIS, AGAR GEL
BASE SEQUENCE
RNA, MESSENGER