Avtor/Urednik		Geršak, B; Trobec, R; Krisch, I
Naslov		Loss of endothelium mediated vascular relaxation as a response to various clamping pressure: part 1. A pharmacological study
Tip		članek
Vir		Panminerva Med
Vol. in št.		Letnik 40, št. 4
Leto izdaje		1998
Obseg		str. 280-5
Jezik		eng
Abstrakt		Backgroudn. The contracion/relaxation response of thoracic aortal rings clamped with two clamping pressures to KCI, noradrenaline and carbachol was studied. Methods. Clamp A had the tip pressure PA=0.60 N/mm2 and clamp B PB=5.16N/mm2. In fifteen Wistar albino rats, wighing 328+-19 g (mean+-SD) the thoracic aorta was occluded for 15 minutes and then three vascular rings (2mm wide) were excised. The proximal unclamped ring served as a control. From distal rings the diameter of aorta was calculated from their circumference 1.6+-0.01 mm(n=15, dmin=1.15mm, dmax=1.70mm). The rings were challenged with cumulative additions of KCI (10-80 mmol/l) to measure the contraction. Then cumulative relaxation to carbachol (0.01-100 micromol) as a response to noradreneline precontraction (0.1 micromol/l) was determined. Results. A significant loss (p<0.05) of vascular relaxation in all clamped rings (clamped with PA and PB clamping pressures) was seen. No significant differences (p>0.05) were observed for contaction between clamped and control rings clamped with clamp A, however the rings clamped with clamp B showed a significant reduction in contraction (p<0.05). No significant differences were seen from control rings between groups A and B (p>0.05), or from clamped rings between groups A and B (p>0.05) for both the contastion and relaxation part of experiments. Conclusions. Endothelial vascular layers are much more susceptible to pressure injuries than was previously belived.
Deskriptorji		AORTA, THORACIC CONSTRICTION ENDOTHELIUM, VASCULAR VASODILATION RATS, WISTAR DISEASE MODELS, ANIMAL MUSCLE, SMOOTH, VASCULAR NOREPINEPHRINE CARBACHOL VASOCONSTRICTION