Avtor/Urednik     Fležar, Matjaž
Naslov     Citosolna aktivnost kalcija in pretoki klorida v epitelnih in gladkomišičnih celicah v kulturi
Prevedeni naslov     Cytosolic activity of calcium and the flux of chloride in epithelial and smooth muscle cells
Tip     monografija
Kraj izdaje     Ljubljana
Založnik     Medicinska fakulteta
Leto izdaje     1999
Obseg     str. 152
Jezik     slo
Abstrakt     Presenting articles, describing the research performed on living cells in cell culture and testing animals, are designed to delineate causal relationship between chloride and calcium activity. The presenting model contributed new knowledge about the applicability of potential drugs for treatment of cystic fibrosis and asthma. In cystic fibrosis, the abnormal regulation of chloride and calcium activity in the cell is caused by genetic defect. In asthma, both ions are directly involved in mechanisms of cell contraction and relaxation. The basic purpose of this work is to highlight the role of extracellulary applied agonist ATP on calcium signaling and chloride secretion that is defective in cystic fibrosis due to genetic defect. Beside that we try to highlight . the role of chloride channel antagonists that produce their effect through regulation of smooth muscle contraction. In the First Chapter we showed an increase in intracellular calcium activity after stimulation with ATP by the method of fluorescence digital microscopy. We tried to link this event with greater efflux of chloride in epithelial cells, used to study ion transport mechanisms in cystic fibrosis. The ATP applied extracellulary was shown to act through P2 purinergic receptor stimulation. The results of this Chapter were the basis for the Second Chapter, where we showed that ATP exerts harmfull effects on human neutrophils by means of stimulated secretion of elastase. Beside that it increases airway resistance and stimulates secretion from the lungs of a normal rat after intratracheal instillation. In the Third Chapter we describe subtypes of histamine receptors present on guinea pig tracheal smooth muscle cells and the effect of dexamethasone on cAMP level, that regulates the relaxation of these cells. The Fourth Chapter describes the decrease in Ca2+Ic activity in presence of chloride channel antagonists (NPPB and cpd 149) in smooth muscle cells and decreased synthesis of both IP2 and IP3 .
Deskriptorji     CYSTIC FIBROSIS
ASTHMA
DISEASE MODELS, ANIMAL
CALCIUM
CHLORIDES
GUINEA PIGS
RATS
CELLS, CULTURED
MUSCLE, SMOOTH
TRACHEA
DEXAMETHASONE
ADENOSINE TRIPHOSPHATE
RECEPTORS, HISTAMINE
CYCLIC AMP