Avtor/Urednik		Milačič, Radmila; Benedik, Miha
Naslov		Determination of trace elements in a large series of spent peritoneal dialysis fluids by atomic absorption spectrometry
Tip		članek
Vir		J Pharm Biomed Anal
Vol. in št.		Letnik 18
Leto izdaje		1999
Obseg		str. 1029-35
Jezik		eng
Abstrakt		An analytical procedure is reported for the determination of six elements in a large series of spent dialysis fluid samples. Determinations of aluminium, chromium, copper, manganese and iron were made by electrothermal atomic absorption spectrometry (ETAAS) with Zeeman background correction, while yinc was analysed by flame atomic absorption spectrometry (FAAS). Because of the complex matrix with high salt content and a high content of proteins, the measurement parameters were optimised for ezch particular element determined by ETAAS. The samples were collected in polyethylene eppendorf cups and stored in a freezer at -20 degree C. When the lements were determined by ETAAS the standard addition method was applied in the calibration procedure. The sample (10 micro l) was injecred into a cuvette and cereful drying and long ashing of samples at temperatures between 850 and 1000 degree C performed. Triton X-100 was added before each determination to reduce the matrix effects of the proteins. Zinc was determined by FAAS in an air-acetylene flame under the usual recommended procedure, calibrating with aqueous standards. The limits of detection (3 sigma) were 1.0 micro g 1-1 for aluminium, 0.20 micro g 1-1 for chromium, 0.40 micro g 1-1 for copper, o.20 micro g 1-1 for manganese, 0.50 micro 1-1 for iron and 5.0 micro g 1-1 for zinc. The reproducibility of the measurements for aluminium, copper, iron and yinc was better than +- 3.0%. It was worse for manganese and chromimum (+- 6.0 and +-12.0%, respectively). since these two elements were present in very low concentrations in all the samples analysed.
Deskriptorji		PERITONEAL DIALYSIS, CONTINUOUS AMBULATORY DIALYSIS SOLUTIONS TRACE ELEMENTS ALUMINUM CHROMIUM COPPER MANGANESE IRON SPECTROPHOTOMETRY, ATOMIC ABSORPTION POLYETHYLENE GLYCOLS