Avtor/Urednik		Petrovec, M; Sumner, JW; Nicholson, WL; Childs, JE; Strle, F; Barlič, J; Lotrič-Furlan, S; Avšič-Županc, T
Naslov		Identity of ehrlichial DNA sequences derived from Ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in Slovenia
Tip		članek
Vir		J Clin Microbiol
Vol. in št.		Letnik 37, št. 1
Leto izdaje		1999
Obseg		str. 209-10
Jezik		eng
Abstrakt		Adult ixodes ricinus (Acari: Ixodidae) ticks collected near Ljubljana, Slovenia, were tested for the agent of human granulocytic ehrlichiosis (HGE) by using PCR assays based on the 16S rRNA gene. Three (3.2%) of 93 ticks were found to contain granulocytic ehrlichiae. Nucleotide sequences of portions of the bacterial groESL heat shock operon amplified from these ticks were identical or nearly (99.8%) identical to those previously determined ror human patients with HGE from Slovenia, providing additional evidence that the ticks were infected with the HGE agent. This study identified I. ricinus a sthe likely vector for these ehrlichial pathogens of humans in this part of Europe.
Deskriptorji		EHRLICHIOSIS IXODES RNA, RIBOSOMAL, 16S POLYMERASE CHAIN REACTION BASE SEQUENCE SEQUENCE ANALYSIS, DNA ARACHNID VECTORS