Avtor/Urednik		Ihan, Alojz
Naslov		A correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cells
Prevedeni naslov		Korelacija med NK citolitičnim in BLT esteraznim testom pri določevanju aktivnosti NK celic stimuliranih s tumorskimi celicami
Tip		članek
Vir		Radiol Oncol
Vol. in št.		Letnik 33, št. 1
Leto izdaje		1999
Obseg		str. 27-33
Jezik		eng
Abstrakt		We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we used buffy-coat isolated NK cells or peripheral blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK test results and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test results.
Izvleček		Z meritvami encimske aktivnosti N-benzyloxycarboxy-L-lizinske esteraze smo preiskovali eksoitozo celic NK. Celice NK smo osamili iz levkocitnega koncentrata (Buffy coat) oz. venske krvi. Eksocitozo smo sprožili s kombinacijo PMA/ionomcin ali s tumorskimi celicami K542. Ob stimulaciji celic NK s tumorskimi celicami (K542) smo opazovali značilno soodvisnost (Cor=0.H4) med rezultati citolitične aktivnosti celic NK in med izmerjenimi encimskimi aktivnosti N-benzv- loxycarboxy-L-lizinske esteraze (BLT test). Ugotavljamo, da BLT test omogoča nadrobnej5i študij dogodkov, ki se odvijajo med citotoksično aktivnostjo celic NK.
Deskriptorji		KILLER CELLS, NATURAL EXOCYTOSIS LYMPHOCYTES ESTERASES SERINE TUMOR CELLS, CULTURED CYTOTOXICITY TESTS, IMMUNOLOGIC FLOW CYTOMETRY CELL DEGRANULATION