Avtor/Urednik     Verovnik, Rudi; Trontelj, Peter; Sket, Boris
Naslov     Genetic differentiation and species status within the snail leech Glossiphonia complanata aggregate (Hurudinea: Glossiphoniidae) revealed by RAPD analysis
Tip     članek
Vir     Arch Hydrobiol
Vol. in št.     Letnik 144, št. 3
Leto izdaje     1999
Obseg     str. 327-38
Jezik     eng
Abstrakt     Randomly amplified polymorphic DNA (RAPD) markers were employed to analyse genetic differrentation and taxonomic structure within the snail leech Glossiphonia complanata species aggregate. There morphological types of snail leeches, whose taxonomic validity was formerly mainly distrusted, occur throughout Europe. Six individuals of each morphotype from locations in Slovenia were investigated. At two locations all three morphotypes occured syntopically, enabling the determination of reproductive isolation. PCR amplification with eleven decamer random primers yielded 177 reproducible marker fragments, only one of which was monomorphic in all specimens. Genetic similary within morphotypes was substantially higher than within samples from the same locality but consisting of different morphotypes. The estimated nucleotide diversity within morphotypes (0.7-2.1%) was lower than the nucleotide divergence between them (5.1-8.3%). UPGMA clustering and principal coordinate analysis revealed three groups concordant with the identified morphotypes. They were tentatively assigned to the taxa Glossiphonia complanata (LINNAEUS), G. concolor (APATHY) and G. nebulosa KALBE. Reproductive isolation between them is suggested by the presence of RAPD fragments specific to each taxon, and by the absence of common fragments in syntopic populations of the three taxa. This is strong support for the hypotesis that externally very similar and anatomically nearly identical glossiphoniids belong to different biological species.
Deskriptorji     LEECHES
VARIATION (GENETICS)
POLYMORPHISM (GENETICS)
RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE
SLOVENIA