Avtor/Urednik		Tejuca, Mayra; Anderluh, Gregor; Maček, Peter; Marcet, Ricardo; Torres, Dinora; Sarracent, Jorge; Alvarez, Carlos; Lanio, Maria E; Dalla-Serra, Mauro; Menestrina, Gianfranco
Naslov		Antiparasite activity of sea-anemone cytolysins on Giardia duodenalis and specific targeting with anti-Giardia antibodies
Tip		članek
Vir		Int J Parasitol
Vol. in št.		Letnik 29
Leto izdaje		1999
Obseg		str. 489-98
Jezik		eng
Abstrakt		The killing activity of sea-anemone cytolysins on Giardia duodenalis was investigated. Three different toxins, sticholysin I and II from Stichodactyla helianthus (St I and St II) and equinatoxin II from Actinia equina (EqtII) were all found to be active in an acute test, with a C5o in the nanomolar range (St I, 0.5 nM; St II, 1.6 nM; and EqtII, 0.8 nM). A method to target the cytolysin activity more specifically towards the parasite cells by using anti-Giardia antibodies was then investigated. Parasite cells were sensitised with a primary murine monoclonal or polyclonal antibody followed by a biotinylated secondary anti-mouse-IgG monoclonal antibody. Subsequently, avidin and a biotinylated EqtII mutant were added, either in two separate steps or as a pre-formed conjugate. When the monoclonal antibody was used, the C5o of biotinylated EqtII was 1.3 nM with sensitised cells and 5 nM with non-sensitised cells, indicating a four-fold enhancement of activity with the cell treatment. Treatment with the polyclonal antibody was somehow more effective than with the monoclonal antibody in an acute test. This indicates that sea-anemone cytolysins can efficiently kill Giardia cells, and that it is possible to improve, to a certain extent, the anti-parasite specificity of these toxins with anti-Giardia antibodies. However, the feasibility of this approach "in vivo" remains to be demonstrated.
Deskriptorji		GIARDIA CNIDARIAN VENOMS IMMUNOTOXINS CYTOTOXINS ANTIBODIES SEA ANEMONES