Avtor/Urednik		Poljšak-Prijatelj, Mateja; Ravnikar, Maja; Brzin, Jože; Štrukelj, Borut
Naslov		Cisteine and aspartic proteinase inhibitors are induced in potato by systemic infection with PVY NTN
Tip		članek
Vir		In: Kovacs K, editor. Proceedings of the 4th multinational congress on electron microscopy; 1999 Sep 5-8; Veszprem. Veszprem: University of Veszprem,
Leto izdaje		1999
Obseg		str. 57-62
Jezik		eng
Abstrakt		In our study we observed the presence, distributioon and intensity of accumulation of cysteine and aspartic proteinase inhibitors in shoot tips, leaves and stems of secondary infected potato plants Solanum tuberosum L. cv. Desiree with the aggressive potato virus Y-PVY NTO-SLO, the causative agent of potato tuber necrotic disease (PTNRD). The potato plants were propagated in stem node tissue culture. After 4 weeks apical shoots, leaves and stems were fixed, dehydrated and embedded in methacrylate resin HM20. After immunolabelling using the protein A-gold method on ultrathin sections, the sections were and examined in transmission electron microscope. In systemically infected plants we demonstrated the induction of potato cysteine proteinase inhibitor PCPI 6.6 in all plant parts, multicystatin predominated mainly in the leaves and shoot tips, while the synthesis of aspartic proteinase inhibitors (API) was induced in the shoot tips. These experiments show us that virus infection induces the expression of protein inhibitors differently in different plant parts, implying that these inhibitors are part of potato defence mechanism against virus infection.
Deskriptorji		POTATOES POTYVIRUS CYSTEINE PROTEINASE INHIBITORS ASPARTIC PROTEINASES MICROSCOPY, ELECTRON