Avtor/Urednik		Lagares, Alfonso; Avendano, Carlos
Naslov		An efficient method to estimate cell number and volume in multiple dorsal root ganglia
Tip		članek
Vir		Acta Stereol
Vol. in št.		Letnik 18, št. 2
Leto izdaje		1999
Obseg		str. 185-95
Jezik		eng
Abstrakt		The combined use of the optical fractionator and the rotator was used to estimate the number of neurons and the mean neuronal volume in multiple and/or individual celloidin-embedded dorsal rott ganglia of adult macaque monkeys. The cervical ganglia C4-T2 from both sides were studied. Ganglia from the same side were embedded in a single block and isotropic orientation of each ganglion in the horizontal plane was ensured in order to obtain vertical sections. Sampling, counting and measuring were carried out with the help of an intreactive computer system, and tst grids were provided by the GRID general sterological software pacjage (Olympus Denmark). The mean number of neurons on each side of the C4-T2 set was 236,500, with a moderate variation among animals (CVa=0.13). Of these neurons,42% were A-type and 56% were B-type. Neuron numbers for each ganglion showed important segmental and side differences both within and between animals. Mean neuronal volume (uncorrected for shrinkage) was 29,862 microm3 for cells (CVa=0.13) and 5,062 microm3 for B cells (CVa=0.08), and no side differences were found. Some size distribution was heavily skewed and there was a substantial overlap between the volume distributions of the two cell types.
Deskriptorji		NEURONS GANGLIA, SPINAL CELL COUNT MACACA NEMESTRINA