Avtor/Urednik		Geršak, Ksenija; Tomaževič, Tomaž
Naslov		Subpopulations of human granulosa-luteal cells obtained from gonadotropin - or gonadotropin-releasing hormone agoinst/gonadotropin-treated follicles in vitro fertilization-embryo transfer cycles
Tip		članek
Vir		J Assist Reprod Genet
Vol. in št.		Letnik 16, št. 9
Leto izdaje		1999
Obseg		str. 488-91
Jezik		eng
Abstrakt		Our purpose was to find the differences in granulosa-luteal cells obtained from gonadotropin-versus gonadotropin-releasing hormone (GnRH) agonist-gonadotropin-treated follicles in in vitro fertilization-embryo transfer (IVF-ET) cycles. Methods. Granulosa-luteal cells were obtained from 45 follicles of women undergoing IVF-ET with gonadotropin releasing hormone (GnRH) agonist and human menopusal gonadotropin releasing hormone (GnRH) agonist and huaman menopausal gonadotropin (hMG) and from 45 follicles of women with HMG IVF-ET cycles. Subpopulations of granulosa-luteal cells were observed by computerized image analysis in which human chorionic gonadotropin (HCG) was localized using immunoperoxidase staining. Results. The luteinized granulosa-luteal cells from hMG-treated follicles were larger than those from GnRH agonist/hMG-treated follicles. The hMG-treated follicles contained more hCG-stained cells, particularly those with cytoplasmic hCG localization. Conclusions. We found differences in morphometric characteristic and hCG localization in granulosa-luteal cells obtained from hMG-versus GnRH agonist/hMG-treated follicles. We presume that the result indicate and importance of luteal-phase support on the clinical pregnancy rate in GnRH agonist/hMG-treated IVF-Et cycles.
Deskriptorji		FERTILIZATION IN VITRO EMBRYO TRANSFER OVULATION INDUCTION MENOTROPINS GONADORELIN PREGNANCY GRANULOSA CELLS LUTEIN CELLS IMAGE PROCESSING, COMPUTER-ASSISTED GONADOTROPINS, CHORIONIC IMMUNOENZYME TECHNIQUES