Avtor/Urednik		Vitalis, TZ; Kern, I; Croome, A; Behzad, H; Hayashi, S; Hogg, JC
Naslov		The effect of latent adenovirus 5 infection on cigarette smoke-induced lung inflammation
Tip		članek
Vir		Eur Respir J
Vol. in št.		Letnik 11, št. 3
Leto izdaje		1998
Obseg		str. 664-69
Jezik		eng
Abstrakt		The aim of this study was to test the hypothesis that latent adenovirus (Ad) 5 infection increases the lung inflammation that follows a single acute exposure to cigarette smoke. A recently developed model of latent adenoviral infection in guineapigs was used. Twelve animals were infected with Ad5 (10 8 plaque-forming units) and 12 animals were sham-infected. Thirty five days later six Ad5-infected and six sham-infected animals were exposed to the smoke from five cigarettes. The remaining animals were used as controls for both infection and smoking. As markers of inflammation, the volume fraction of macrophages, T-lymphocytes, neutrophils and eosinophils were measured by quantitative histology. We found that latent Ad5-infection alone, doubled the number of macrophages in the lung parenchyma and that smoking alone, doubled the volume fraetion of neutrophils in the airway wall and the volume fraction of macrophages in the lung parenchyma. Neither viral infection nor smoking, alone, had an effect on T-lymphocytes or eosinophils. However, the combination of viral infection and smoking doubled the T lymphocyte helper cells and quadrupled the volume fraction of macrophages in the lung parenchyma. We conclude that in guinea-pigs, latent adenovirus 5 infection increases the inflammation that follows a single acute exposure to cigarette smoke, by increasing the volume fraction of macrophages and T-lymphocyte helper cells.
Deskriptorji		ADENOVIRIDAE INFECTIONS BRONCHIOLITIS, VIRAL LUNG ADENOVIRUS E1A PROTEINS SMOKE GUINEA PIGS MACROPHAGES POLYMERASE CHAIN REACTION T-LYMPHOCYTE SUBSETS TOBACCO SMOKE POLLUTION