Avtor/Urednik | Zrimšek, P; Kos, J; Pinter, L; Drobnič-Košorok, M | |
Naslov | Detection by ELISA of the humoral immune response in rabbits naturally infected with Trichophyton mentagrophytes | |
Tip | članek | |
Vir | Vet Microbiol | |
Vol. in št. | Letnik 70 | |
Leto izdaje | 1999 | |
Obseg | str. 77-86 | |
Jezik | eng | |
Abstrakt | An indirect enzyme linked immunosorbent assay (ELISA) was developed and its diagnostic potential evaluated for rabbits infected by Trichophyton mentagrophytes. Within-run and between-run coefficient of variance varied from 2.3 to 7.7% and from 5.9 to 8.5%, respectively, indicating satisfactory reproducibility of the ELISA. There was no significant cross-reaction with antigens of Microsporum canis, Malassezia pachydermatis and Aspergillus fumigatus. The level of specific IgG to Trichophyton mentagrophytes was measured in sera of 25 11-week-old and 12 younger infected rabbits. There was no significant difference in the IgG level between 12 5-week-old infected rabbits and controls (p = 0.38). The antibody response was higher in 12 7-week-old rabbits compared with controls (p = 0.001 ). The IgG level in 25 11-week-old rabbits differed from the controls very significantly (p < 0.0001). Increased specific IgG in 11- week-old rabbits exhibited 96% sensitivity and 94% specificity. Predictive values of a positive and a negative test were 96 and 94%, respectively. Western immunoblotting associated three protein bands (21.5, 31, 44 kDa) with Trichophyton mentagrophytes infection. | |
Deskriptorji | TINEA TRICHOPHYTON ENZYME-LINKED IMMUNOSORBENT ASSAY RABBITS IGG BLOTTING, WESTERN MICROSPORUM MALASSEZIA ASPERGILLUS FUMIGATUS CROSS REACTIONS |