Avtor/Urednik		Zrimšek, P; Kos, J; Pinter, L; Drobnič-Košorok, M
Naslov		Detection by ELISA of the humoral immune response in rabbits naturally infected with Trichophyton mentagrophytes
Tip		članek
Vir		Vet Microbiol
Vol. in št.		Letnik 70
Leto izdaje		1999
Obseg		str. 77-86
Jezik		eng
Abstrakt		An indirect enzyme linked immunosorbent assay (ELISA) was developed and its diagnostic potential evaluated for rabbits infected by Trichophyton mentagrophytes. Within-run and between-run coefficient of variance varied from 2.3 to 7.7% and from 5.9 to 8.5%, respectively, indicating satisfactory reproducibility of the ELISA. There was no significant cross-reaction with antigens of Microsporum canis, Malassezia pachydermatis and Aspergillus fumigatus. The level of specific IgG to Trichophyton mentagrophytes was measured in sera of 25 11-week-old and 12 younger infected rabbits. There was no significant difference in the IgG level between 12 5-week-old infected rabbits and controls (p = 0.38). The antibody response was higher in 12 7-week-old rabbits compared with controls (p = 0.001 ). The IgG level in 25 11-week-old rabbits differed from the controls very significantly (p < 0.0001). Increased specific IgG in 11- week-old rabbits exhibited 96% sensitivity and 94% specificity. Predictive values of a positive and a negative test were 96 and 94%, respectively. Western immunoblotting associated three protein bands (21.5, 31, 44 kDa) with Trichophyton mentagrophytes infection.
Deskriptorji		TINEA TRICHOPHYTON ENZYME-LINKED IMMUNOSORBENT ASSAY RABBITS IGG BLOTTING, WESTERN MICROSPORUM MALASSEZIA ASPERGILLUS FUMIGATUS CROSS REACTIONS